ATM is required for efficient transduction of primary fibroblasts. (A) HIV-1 transduction of the lacZ marker as measured by detecting lacZ reporter activity in infected A-T fibroblast and control fibroblast cells. Cells were infected with an HIV-1-based vector carrying the lacZ reporter at an m.o.i. of 0.3. Infected cells were stained overnight using a β-galactosidase assay at five and seven dpi and transduced cells counted. (B) Light microscopic images from the same experiment as in A, taken 4 dpi. (C) A-T and control fibroblast infections with the HIV based vector carrying the EGFP marker. Cells were evaluated by flow cytometry at multiple time points (2–7 dpi). Results from 7 dpi are shown. Histograms of mock-infected control cells (left), control cells infected at m.o.i. of 0.1 (middle), and infected A-T cells are shown. The error bars represent standard deviation, p = 0.0014 for 5 dpi and 0.027 for 7 dpi.
Smith et al. Virology Journal 2008 5:11 doi:10.1186/1743-422X-5-11