NBS1 is required for efficient HIV-1 transduction of lymphoid cells. EBV-transformed NBS1-deficient B-lymphoid cells (GM15818) and matched control EBV-transformed cells (GM15817) were infected with the HIV-1-based vector carrying the EGFP reporter and then assayed 7 dpi by FACS to quantify reporter gene expression. At an m.o.i. of 0.1, control lymphocytes were infected at a rate of 7.29%, while only 2.64% of NBS lymphocytes were infected.
Smith et al. Virology Journal 2008 5:11 doi:10.1186/1743-422X-5-11