Reintroduction of normal NBS1 cDNA into NBS1-deficient cells restores HIV-1 transduction efficiency. NBS1-deficient cells were transfected with a plasmid encoding normal NBS1 cDNA or an empty vector plasmid. One day post-transfection, cells were infected with the HIV-1-based vector carrying the lacZ reporter. Cells were then stained eight days post-infection using a β-galactosidase assay and transduced (blue) cells were counted. c – control (cells transfected) with the empty vector, NBS1 – cells transfected with the plasmid carrying the normal NBS1 gene. The error bars represent standard deviation, p = 0.037.
Smith et al. Virology Journal 2008 5:11 doi:10.1186/1743-422X-5-11