Permissivity of bovine cell lines for myxoma virus. Bovine cells were maintained in DMEM (KOP-R and BT) or MEM (MDBK) supplemented with 10 % foetal calf serum (FCS). A. Virus production in different bovine cell lines over three passages. Cells were first inoculated with either T1 (top) or SG33 (bottom) MYXV strain at a m.o.i. of 1, washed, cultured in DMEM, 5 % FCS for 3 days and frozen (P1). In subsequent infections, 1/5 of the material from the previous frozen culture was used for infection (P2 and P3). Titers were determined by serial dilution-titration on RK13 cells. The values correspond to a mean of at least two independent experiments. Error bars correspond to the standard error of the mean. B. Rabbit and bovine cells were infected with the T1-TK::LacZ (m.o.i. of 0.1 and 1). Twenty-four hours p.i., they were fixed with 2.5 % glutaraldehyde for 15 minutes at room temperature and stained with 2 mg/ml X-Gal in 2 mM MgCl2, 5 mM K4Fe(CN)6.3H2O, 5 mM K3Fe(CN)6 in PBS for 4–10 hours and observed by microscopy. Microscope: Leica; Magnification: 100.
Pignolet et al. Virology Journal 2007 4:94 doi:10.1186/1743-422X-4-94