In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strains
1 Laboratory « Interactions Hôtes-Virus et Vaccinologie », UMR 1225 INRA-ENVT, Ecole Nationale Vétérinaire de Toulouse, 23 chemin des capelles, Toulouse F-31076, France
2 laboratory « Résistome des ruminants », UMR 1225 INRA-ENVT, Ecole Nationale Vétérinaire de Toulouse, 23 chemin des capelles, Toulouse F-31076, France
3 Centre de Microscopie Electronique Appliquée à la Biologie, Faculté de Médecine de Rangueil, 133 route de Narbonne, Toulouse, F-31062, France
Virology Journal 2007, 4:94 doi:10.1186/1743-422X-4-94Published: 27 September 2007
Myxoma virus (MYXV), a leporide-specific poxvirus, represents an attractive candidate for the generation of safe, non-replicative vaccine vector for non-host species. However, there is very little information concerning infection of non-laboratory animals species cells with MYXV. In this study, we investigated interactions between bovine cells and respectively a wild type strain (T1) and a vaccinal strain (SG33) of MYXV. We showed that bovine KOP-R, BT and MDBK cell lines do not support MYXV production. Electron microscopy observations of BT-infected cells revealed the low efficiency of viral entry and the production of defective virions. In addition, infection of bovine peripheral blood mononuclear cells (PBMC) occurred at a very low level, even following non-specific activation, and was always abortive. We did not observe significant differences between the wild type strain and the vaccinal strain of MYXV, indicating that SG33 could be used for new bovine vaccination strategies.