Interferon action on the IRES-mediated translational inhibition was prevented in cured resistant cell lines. Effect of interferon on IRES-GFP translation regulation was examined using one cured sensitive and nine resistant Huh-7 cell lines. Cells were transfected with IRES-GFP plasmid and treated with 1000 IU/ml interferon alpha. After 24 hours, GFP expression was recorded. Cured cells prepared from resistant clones unable to activate interferon signaling and no inhibition of HCV IRES was seen.
Hazari et al. Virology Journal 2007 4:89 doi:10.1186/1743-422X-4-89