Figure 5.

Coimmunoprecipitation of proteins that interact with ECTV-PH. His-tagged ECTV-PH was immunoprecipitated with mouse monoclonal anti-His antibodies and Protein G-Plus agarose along with any bound proteins from a BS-C-1 cell lysate. Proteins were separated by SDS-PAGE and stained with Coomassie blue. Lane 1, control immunoprecipitation; lysate contained no His-tagged ECTV-PH. Lane 2, proteins isolated from immunoprecipitation on cells expressing His-tagged ECTV-PH. Three bands at 16 kDa, 38 kDa and 84 kDa were excised from the gel and identified by mass spectrometry as indicated; a fourth band at 28 kDa was identified as a dimer of ECTV-PH in a western blot.