Table 1

Summary of the mutations introduced into the rHPIV1 genomea.
Gene
 Mutationb
 ORF
 nt changes wt → mutant c
 Type of mutation
 Codon position
 Amino acid change
 # nt changes for reversion to wt
P/C
 R84G
 C
 AGA → GGA
 point
 84
 R → G
 1


P
 GAG → GGG
 point
 87
 E → G
 1

Δ170d
 C
 AGG GAT TTC → AGC
 deletion
 168–170
 RDF → S (D deletion; 3 nt deletions in the flanking R-F codons results in a S substitution)
 6 (insertions)d


P
 GGA TTT→ deletion
 deletion
 172–173
 GF deletion
 6 (insertions)
HN
 T553A
 HN
 ACC → GCC
 point
 553
 T → A
 1
L
 Y942Ae
 L
 TAT→ GCG
 point
 942
 Y → A
 3e

Δ1710–11d
 L
 GCT GAG→ deletion
 deletion
 1710–11
 AE deletion
 6 (insertions)d

a HPIV1 strain Washington/1964, GenBank accession no. NC_003461.

b The nomenclature used to describe each mutation indicates the wt amino acid, the codon position and the new amino acid, or the position of the deletion (Δ), with respect to the C, HN or L protein.

c The nucleotides (nt) affected by substitution or deletion are shown underlined and in bold type.

d Designed for increased genetic stability by use of a deletion. Deletions involved six nt to conform to the rule of six [20].

e Designed for increased genetic stability by the use of a codon that differs by three nucleotides from codons yielding a wild type assignment.

Bartlett et al. Virology Journal 2007 4:67   doi:10.1186/1743-422X-4-67