PCR Screening and Sequencing of Randomly Amplified Coxsackie Virus A7 cDNA. Randomly amplified cDNA from Coxsackie Virus A7-infected plasma was shot-gun ligated into pCR 2.1-TOPO and competent E. coli were transformed. Resultant colonies were screened for the presence of recombinant plasmid DNA (A) and plasmid DNA from positive colonies was then purified and sequenced (B). Sequence data from recombinant plasmid #7 (see *) was aligned to all sequence data in the Non-Redundant NCBI Database using the NCBI Nucleotide-Nucleotide BLAST (blastn) Search Algorithm (version 2.2.8).
Clem et al. Virology Journal 2007 4:65 doi:10.1186/1743-422X-4-65