5'-VVVVVVVVAA-3' Primers Enable Random Multiplex Amplification of DNA from Human Plasma Inoculated with Adenovirus Type 17. 1 ml of human plasma was inoculated with 0–5 μl of suspended Adenovirus Type 17 (ATCC), filtered and incubated for 2 hours with nucleases. Remaining nucleic acids were purified with the QiaAmp UltrasSens Virus Kit (Qiagen) and subjected to the random multiplex PCR with 5'-VVVVVVVVAA-5' primers as detailed in the Methods section. Amplicons were then visualized on an ethidium bromide impregnated agarose gel.
Clem et al. Virology Journal 2007 4:65 doi:10.1186/1743-422X-4-65