Filtration and Nuclease Treatment Improves Viral DNA Amplification by Removing Host DNA and mRNA. Human plasma was inoculated with 1 μl of an Adenovirus Type 17 suspension, filtered and incubated for 2 hrs with either 10 u DNAse or 5 u RNAse as indicated. Remaining nucleic acids were purified with the QiaAmp UltrasSens Virus Kit and subjected to 1st strand cDNA synthesis and 50 cycles PCR using primers specific for either Adenovirus Type 17 or human β-Actin DNA (upper band; primers cross an intron) or cDNA (lower band). Amplicons were then visualized on an ethidium bromide impregnated agarose gel.
Clem et al. Virology Journal 2007 4:65 doi:10.1186/1743-422X-4-65