Figure 1.

Filtration and Nuclease Treatment Improves Viral DNA Amplification by Removing Host DNA and mRNA. Human plasma was inoculated with 1 μl of an Adenovirus Type 17 suspension, filtered and incubated for 2 hrs with either 10 u DNAse or 5 u RNAse as indicated. Remaining nucleic acids were purified with the QiaAmp UltrasSens Virus Kit and subjected to 1^st strand cDNA synthesis and 50 cycles PCR using primers specific for either Adenovirus Type 17 or human β-Actin DNA (upper band; primers cross an intron) or cDNA (lower band). Amplicons were then visualized on an ethidium bromide impregnated agarose gel.