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Replication of rDEN1/4(ME) chimeric viruses in Aedes aegypti |
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| Virus1 |
No. mosquitoes |
Body2 |
Head2 |
% Dissemination3 |
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|
|
|
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| % infected |
Mean virus titer (log10 PFU/body) |
% infected |
Mean virus titer (log10 PFU/head) |
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|
|
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| DEN1 |
24 |
88 |
4.5 ± 0.1 |
88 |
4.7 ± 0.1 |
100 |
| DEN4 |
24 |
88 |
3.0 ± 0.2 |
71 |
2.7 ± 0.3 |
81 |
| rDEN1/4(ME) |
24 |
63 |
2.4 ± 0.3 |
42 |
3.0 ± 0.4 |
67 |
| rDEN1/4Δ30(ME) |
24 |
0 |
< 0.4 |
0 |
< 0.4 |
0 |
|
1 Assuming a 2 ul bloodmeal, 4.3 log10 PFU virus of each virus were ingested. 2 Presence of virus in mosquito homogenates was determined by plaque assay in Vero cells. Mean virus titer was determined using values only from samples with detectable virus. 3 Calculated as no. infected heads/no. infected bodies. | ||||||
Blaney et al. Virology Journal 2007 4:23 doi:10.1186/1743-422X-4-23 |
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