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Replication and immunogenicity of rDEN1/4 chimeric viruses in rhesus monkeys. |
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| Virus1 |
No. of monkeys |
% of monkeys with viremia |
Mean no. of viremic days per monkey |
Mean peak virus titer2 (log10 PFU/ml ± SE) |
Geometric mean serum neutralizing antibody titer (reciprocal dilution)3 |
Post-challenge5 |
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| Day 0 |
Day 28 |
% sero-conversion4 |
% of monkeys with viremia |
Mean peak virus titer2 (log10 PFU/ml ± SE) |
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| DEN1 Puerto Rico/94 |
4 |
100 |
2.8 |
2.0 ± 0.1 |
< 5 |
103 |
100 |
0 |
< 1.0 |
| rDEN1/4 (CME) |
4 |
100 |
2.0 |
1.5 ± 0.1 |
< 5 |
56 |
100 |
0 |
< 1.0 |
| rDEN1/4Δ30 (CME) |
4 |
50 |
0.5 |
1.1 ± 0.1 |
< 5 |
< 5 |
0 |
75 |
1.7 ± 0.3 |
| rDEN1/4 (ME) |
4 |
100 |
4.0 |
2.1 ± 0.1 |
< 5 |
65 |
100 |
0 |
< 1.0 |
| rDEN1/4Δ30 (ME) |
6 |
50 |
0.7 |
1.1 ± 0.2 |
< 5 |
23 |
66 |
0 |
< 1.0 |
| Mock infected |
2 |
0 |
0 |
< 1.0 |
< 5 |
< 5 |
0 |
100 |
2.1 ± 0.2 |
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1 Groups of rhesus monkeys were inoculated subcutaneously on day 0 with 5.0 log10 PFU of the indicated virus in a 1 ml dose. Serum was collected daily for 10 days to detect viremia and on day 28 for determination of neutralizing antibodies. 2 Virus titer in serum was determined by plaque assay in Vero cells. 3 Plaque reduction (60%) neutralizing antibody titers were determined using DEN1 Puerto Rico/94 as test virus. 4 Percentage of monkeys with a four-fold or greater rise in neutralizing antibody levels between day 0 and 28. 5 Monkeys were challenged after 35 days with DEN1 (Puerto Rico/94) administered subcutaneously in a 1 ml dose containing 5.0 log10 PFU. Serum was collected daily for 10 days to detect viremia. | |||||||||
Blaney et al. Virology Journal 2007 4:23 doi:10.1186/1743-422X-4-23 |
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