Figure 1.

Co-staining of HSV-2 gB or gD with HIV-1 gp160. HIV-1 infected P4P cells were either superinfected by HSV-2 (A-F) or transfected by a plasmid coding for gB (G-I) or gD (J-L). Cells were stained by immunofluorescence for the co-detection of gp160 and gB (A-C, G-I) or gp160 and gD (D-F, J-L), using human polyclonal anti-gp160 followed by FITC-labelled F(ab')2 rabbit anti-human IgG giving a green staining for HIV-1 gp160 and mouse Mabs against gB or gD followed by a TRITC-labelled rabbit anti-mouse IgG giving a red staining for HSV-2 gB or HSV-2 gD.