SIV escape mutants in rhesus macaques vaccinated with NEF-derived lipopeptides and challenged with pathogenic SIVmac251

doi:10.1186/1743-422X-3-65

Virology Journal

Volume 3

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Villefroy P
Letourneur F
Coutsinos Z
Mortara L
Beyer C
Gras-Masse H
Guillet JG
Bourgault-Villada I

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Letourneur F
Coutsinos Z
Mortara L
Beyer C
Gras-Masse H
Guillet JG
Bourgault-Villada I
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SIV escape mutants in rhesus macaques vaccinated with NEF-derived lipopeptides and challenged with pathogenic SIVmac251

Pascale Villefroy¹^,2^,3^,4^* , Franck Letourneur¹^,2^,3^,4^* , Zoe Coutsinos¹^,2^,3^,4 , Lorenzo Mortara¹^,2^,3^,4^,14 , Christian Beyer⁵^,6^,7 , Helene Gras-Masse⁸^,9^,10^,11 , Jean-Gerard Guillet¹^,2^,3^,4 and Isabelle Bourgault-Villada¹^,2^,3^,4^,12^,13

¹Institut Cochin, Département d'Immunologie, Hôpital Cochin, 27, rue du Faubourg Saint-Jacques, Paris, F-75014, France

²INSERM U567, Paris, F-75014, France

³CNRS UMR 8104, Paris, F-75014, France

⁴Université Paris 5, Faculté de Médecine René Descartes, UM3, F-75014, France

⁵Institut de Virologie de la Faculté de Médecine, 3 rue Koeberlé, Strasbourg, F-67000, France

⁶INSERM U74, Strasbourg, F-67000, France

⁷Université Pasteur de Strasbourg I, Strasbourg, F-67000, France

⁸Institut de Biologie de Lille, Laboratoire Synthèse, Structure et Fonction des Biomolécules, 1 rue du Professeur Calmette, BP 447, F-59021 Lille Cedex, France

⁹URA CNRS 1309, F-59021 Lille Cedex, France

¹⁰Université de Lille II, F-59021 Lille Cedex, France

¹¹Institut Pasteur de Lille, F-59021 Lille Cedex, France

¹²Assistance Publique-Hôpitaux de Paris, Service de Dermatologie, Hôpital Ambroise Paré, 9 avenue Charles de Gaulle, F-92104 Boulogne, France

¹³Université de Versailles Saint Quentin en Yvelines, Versailles Cedex, F-78035, France

¹⁴Department of Clinical and Biological Sciences, School of Medicine, University of Insubria, Varese, Italy

author email corresponding author email* Contributed equally

Virology Journal 2006, 3:65doi:10.1186/1743-422X-3-65


Published:	31 August 2006

Abstract

Background

Emergence of viral variants that escape CTL control is a major hurdle in HIV vaccination unless such variants affect gene regions that are essential for virus replication. Vaccine-induced multispecific CTL could also be able to control viral variants replication. To explore these possibilities, we extensively characterized CTL responses following vaccination with an epitope-based lipopeptide vaccine and challenge with pathogenic SIVmac251. The viral sequences corresponding to the epitopes present in the vaccine as well as the viral loads were then determined in every macaque following SIV inoculation.

Results

In most cases, the emergence of several viral variants or mutants within vaccine CTL epitopes after SIV challenge resulted in increased viral loads except for a single macaque, which showed a single escape viral variant within its 6 vaccine-induced CTL epitopes.

Conclusion

These findings provide a better understanding of the evolution of CD8+ epitope variations after vaccination-induced CTL expansion and might provide new insight for the development of an effective HIV vaccine.