|
Expression profiles of 17 housekeeping genes in a panel of cell lines |
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| B95.8 |
BJAB |
Namalwa |
Raji |
Akata |
Jijoye |
P3HR1 |
All cell lines |
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|
|
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| High expression |
Mean |
SD |
CV |
Mean |
SD |
CV |
Mean |
SD |
CV |
Mean |
SD |
CV |
Mean |
SD |
CV |
Mean |
SD |
CV |
Mean |
SD |
CV |
Mean |
SD |
CV |
|
|
||||||||||||||||||||||||
| RPL37A |
0.36 |
0.05 |
0.15 |
0.33 |
0.02 |
0.06 |
1.31 |
0.18 |
0.14 |
0.27 |
0.21 |
0.76 |
1.69 |
0.02 |
0.01 |
2.05 |
0.01 |
0.01 |
1.35 |
0.05 |
0.04 |
1.10 |
0.68 |
0.62 |
| KIAA0220 |
0.06 |
0.01 |
0.12 |
2.33 |
0.60 |
0.26 |
1.56 |
0.07 |
0.05 |
2.98 |
0.79 |
0.27 |
1.64 |
0.03 |
0.02 |
2.02 |
0.01 |
0.01 |
1.37 |
0.02 |
0.01 |
1.63 |
0.87 |
0.54 |
| CLU |
1.62 |
0.02 |
0.01 |
0.21 |
0.04 |
0.21 |
0.06 |
0.03 |
0.41 |
n.d. |
0.00 |
0.13 |
0.04 |
0.33 |
0.20 |
0.06 |
0.28 |
0.20 |
0.02 |
0.12 |
0.26 |
0.59 |
2.24 |
|
| MTA2 |
0.00 |
0.01 |
12.49 |
0.02 |
0.02 |
1.13 |
n.d. |
0.00 |
n.d. |
0.00 |
n.d. |
0.00 |
n.d. |
0.00 |
n.d. |
0.00 |
||||||||
| 384D8-2.2 |
0.27 |
0.05 |
0.17 |
0.32 |
0.04 |
0.13 |
0.35 |
0.04 |
0.11 |
n.d. |
0.00 |
0.36 |
0.06 |
0.16 |
0.70 |
0.21 |
0.30 |
0.64 |
0.04 |
0.07 |
0.29 |
0.43 |
1.47 |
|
|
|
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| Constant expression |
Mean |
SD |
CV |
Mean |
SD |
CV |
Mean |
SD |
CV |
Mean |
SD |
CV |
Mean |
SD |
CV |
Mean |
SD |
CV |
Mean |
SD |
CV |
Mean |
SD |
CV |
|
|
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| FTL |
1.49 |
0.08 |
0.05 |
0.20 |
0.01 |
0.05 |
0.10 |
0.05 |
0.54 |
n.d. |
0.00 |
0.33 |
0.12 |
0.36 |
0.51 |
0.25 |
0.49 |
0.75 |
0.10 |
0.13 |
0.45 |
0.59 |
1.31 |
|
| PSMD2 |
0.89 |
0.04 |
0.04 |
2.38 |
0.67 |
0.28 |
1.08 |
0.05 |
0.04 |
0.24 |
0.01 |
0.06 |
1.67 |
0.02 |
0.01 |
1.46 |
0.20 |
0.14 |
1.04 |
0.15 |
0.14 |
1.22 |
0.63 |
0.52 |
| PSMB3 |
1.49 |
0.02 |
0.01 |
2.35 |
0.62 |
0.27 |
1.61 |
0.12 |
0.08 |
0.62 |
0.22 |
0.36 |
1.70 |
0.00 |
0.00 |
2.03 |
0.00 |
0.00 |
1.35 |
0.04 |
0.03 |
1.57 |
0.51 |
0.33 |
| TCLF1 |
0.53 |
0.02 |
0.03 |
0.72 |
0.01 |
0.02 |
1.43 |
0.13 |
0.09 |
0.20 |
0.06 |
0.30 |
0.51 |
0.21 |
0.42 |
1.26 |
0.32 |
0.25 |
1.18 |
0.11 |
0.09 |
0.90 |
0.40 |
0.52 |
| H3F3A |
1.71 |
0.03 |
0.02 |
2.44 |
0.76 |
0.31 |
1.64 |
0.13 |
0.08 |
7.38 |
4.68 |
0.63 |
1.69 |
0.00 |
0.00 |
2.08 |
0.01 |
0.01 |
1.35 |
0.04 |
0.03 |
2.44 |
2.03 |
0.83 |
| PTDSS1 |
1.40 |
0.00 |
0.00 |
2.45 |
0.76 |
0.31 |
1.51 |
0.07 |
0.04 |
4.87 |
1.42 |
0.29 |
0.95 |
0.02 |
0.02 |
1.92 |
0.09 |
0.05 |
1.35 |
0.03 |
0.02 |
1.98 |
1.25 |
0.53 |
| KARS |
1.19 |
0.03 |
0.03 |
2.45 |
0.77 |
0.31 |
1.62 |
0.12 |
0.07 |
6.40 |
3.35 |
0.52 |
1.66 |
0.02 |
0.01 |
2.08 |
0.01 |
0.00 |
1.35 |
0.05 |
0.03 |
2.22 |
1.75 |
0.79 |
| AAMP |
0.57 |
0.01 |
0.02 |
0.62 |
0.01 |
0.01 |
0.59 |
0.04 |
0.07 |
0.13 |
0.04 |
0.31 |
0.55 |
0.07 |
0.12 |
1.00 |
0.18 |
0.18 |
1.29 |
0.02 |
0.01 |
0.71 |
0.35 |
0.50 |
|
|
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| Common |
Mean |
SD |
CV |
Mean |
SD |
CV |
Mean |
SD |
CV |
Mean |
SD |
CV |
Mean |
SD |
CV |
Mean |
SD |
CV |
Mean |
SD |
CV |
Mean |
SD |
CV |
|
|
||||||||||||||||||||||||
| βactin.sense(ACTB) |
1.73 |
0.04 |
0.02 |
1.68 |
0.28 |
0.17 |
1.21 |
0.07 |
0.06 |
2.28 |
0.23 |
0.10 |
0.78 |
0.13 |
0.16 |
1.72 |
0.24 |
0.14 |
1.34 |
0.01 |
0.00 |
1.49 |
0.46 |
0.31 |
| βactin.70 mer(ACTB) |
1.69 |
0.02 |
0.01 |
1.00 |
0.24 |
0.24 |
1.42 |
0.04 |
0.02 |
1.16 |
0.05 |
0.05 |
1.65 |
0.01 |
0.01 |
1.44 |
0.01 |
0.01 |
1.33 |
0.05 |
0.04 |
1.37 |
0.23 |
0.17 |
| c-yes.70 mer |
1.32 |
0.02 |
0.02 |
2.05 |
0.42 |
0.20 |
1.51 |
0.12 |
0.08 |
0.92 |
0.12 |
0.13 |
1.70 |
0.02 |
0.01 |
2.05 |
0.00 |
0.00 |
1.35 |
0.06 |
0.04 |
1.67 |
0.61 |
0.37 |
| c-yes.2 |
0.45 |
0.05 |
0.10 |
0.11 |
0.03 |
0.32 |
n.d. |
0.00 |
n.d. |
0.00 |
0.05 |
0.01 |
0.28 |
0.13 |
0.01 |
0.11 |
0.08 |
0.01 |
0.11 |
|||||
| MHCL.70 mer |
1.73 |
0.05 |
0.03 |
0.48 |
0.13 |
0.27 |
1.57 |
0.07 |
0.05 |
0.80 |
0.01 |
0.01 |
1.69 |
0.02 |
0.01 |
0.04 |
0.04 |
1.18 |
1.27 |
0.02 |
0.02 |
1.10 |
0.62 |
0.55 |
| HMBS.2 |
1.03 |
0.02 |
0.13 |
2.42 |
0.72 |
0.30 |
1.59 |
0.10 |
0.06 |
2.57 |
0.39 |
0.15 |
1.47 |
0.03 |
0.02 |
1.97 |
0.05 |
0.03 |
1.35 |
0.05 |
0.04 |
1.52 |
0.33 |
0.22 |
|
CV: coefficient of variation; n.d.: not detected.; SD: Standard deviation; bold letters indicate gene-sets that were considered to be constantly detectable across cell lines, and were selected to be used as normalization-set in further experiments. Probe sets that showed a CV < 0.6 were selected for the normalization housekeeping gene set. | ||||||||||||||||||||||||
Bernasconi et al. Virology Journal 2006 3:43 doi:10.1186/1743-422X-3-43 |
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