Figure 4.

Immunoprecipitation of HRSV F cysteine mutations. 293T cells were mock transfected (-), transfected with a plasmid expressing beta-galactosidase (b-gal), or plasmids encoding the wild-type (WT) HRSV F protein or various cysteine mutants (listed above lanes), followed by metabolic labeling with [³⁵S]-methionine/cysteine mixture, and immunoprecipitation as described in [44]. The positions of molecular weight size markers are indicated. The positions of the F1 and F2 subunits are indicated with arrows.