T cell distribution in proximity to the luminal surface of human palatine tonsil. Tissue sections were incubated with primary antibodies as indicated below. Positive cells are stained brown. Sections were counterstained with hematoxylin. a: CD3; b: CD4 – large irregular shaped CD4+ cells within follicles are macrophages; c: CD8; d: CD3; e: CD4; f: CD8 – d, e and f represent enlargements of the regions enclosed within boxes in a, b and c respectively; g: H&E staining of tonsillar epithelium; h: enlargement of region enclosed within the box in g; i; independent tonsil section stained with H&E to show surface lymphocytes. Original magnification a, b, c, g: ×100; d, e, f, h, i: ×400.
Kumar et al. Virology Journal 2006 3:25 doi:10.1186/1743-422X-3-25