Figure 2.

The effect of cholesterol depletion from NIH3T3 Targets on CD4-independent HIV-1 Env-mediated fusion. CMFDA-labeled cells (2 × 10⁵ per well) expressing HIV-1_8x (black and light gray bars) or HIV-1_8xV3BAL (dark gray and white bars) Envs were added to the CMTMR-labeled targets expressing cognate co-receptors. The samples were incubated for 6 hrs at 37°C before, after and following cholesterol put-back as described in the legends to Figure 1. Fusion was determined as described in materials and methods.