Figure 4.

Time-course analysis of cellular and viral protein synthesis in cells expressing HCV polyprotein. A: BSC40 cells infected with the recombinant VT7-HCV7.9 virus in the presence (+) or absence (-) of IPTG were metabolically labelled with [³⁵S] Met-Cys Promix (50 μCi/mL) at the indicated times (h.p.i) and analysed by SDS-PAGE (12%) and autoradiography. For comparative purposes, we included a similar inducible recombinant virus but expressing the IBDV mature structural capsid protein VP3 (VT7-VP3). B: Inhibition of VV proteins after expression of HCV. The levels of VV proteins were quantitated from autoradiograms using a BioRad GS700 image densitometer and computer software as suggested by the manufacturer. C: Immunoblot analysis of phospho-eIF-2α-S51 protein levels during the time-course of VT7-HCV7.9 infection. The number appearing in each lane represents the ratio of phospho-eIF-2α-S51 levels in infected cells compared to levels in uninfected cells.