Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections

doi:10.1186/1743-422X-2-7

Virology Journal

Volume 2

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Radonić A
Thulke S
Bae HG
Müller MA
Siegert W
Nitsche A

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Thulke S
Bae HG
Müller MA
Siegert W
Nitsche A
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Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections

Aleksandar Radonić¹ , Stefanie Thulke¹ , Hi-Gung Bae² , Marcel A Müller² , Wolfgang Siegert¹ and Andreas Nitsche²

¹Charité – CCM, Medizinische Klinik II m.S. Hämatologie/Onkologie, Berlin, Germany

²Robert Koch Institut, ZBS 1, Berlin, Germany

author email corresponding author email

Virology Journal 2005, 2:7doi:10.1186/1743-422X-2-7


Published:	10 February 2005

Abstract

Ten potential reference genes were compared for their use in experiments investigating cellular mRNA expression of virus infected cells. Human cell lines were infected with Cytomegalovirus, Human Herpesvirus-6, Camelpox virus, SARS coronavirus or Yellow fever virus. The expression levels of these genes and the viral replication were determined by real-time PCR. Genes were ranked by the BestKeeper tool, the GeNorm tool and by criteria we reported previously. Ranking lists of the genes tested were tool dependent. However, over all, β-actin is an unsuitable as reference gene, whereas TATA-Box binding protein and peptidyl-prolyl-isomerase A are stable reference genes for expression studies in virus infected cells.