Research
Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections
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* Corresponding author: Aleksandar Radonić aleksandar.radonic@charite.de
1 Charité – CCM, Medizinische Klinik II m.S. Hämatologie/Onkologie, Berlin, Germany
2 Robert Koch Institut, ZBS 1, Berlin, Germany
Virology Journal 2005, 2:7 doi:10.1186/1743-422X-2-7
Published: 10 February 2005Abstract
Ten potential reference genes were compared for their use in experiments investigating cellular mRNA expression of virus infected cells. Human cell lines were infected with Cytomegalovirus, Human Herpesvirus-6, Camelpox virus, SARS coronavirus or Yellow fever virus. The expression levels of these genes and the viral replication were determined by real-time PCR. Genes were ranked by the BestKeeper tool, the GeNorm tool and by criteria we reported previously. Ranking lists of the genes tested were tool dependent. However, over all, β-actin is an unsuitable as reference gene, whereas TATA-Box binding protein and peptidyl-prolyl-isomerase A are stable reference genes for expression studies in virus infected cells.