Figure 2.

Cellular localization of HPV16 E6s in 293T (A) and HaCaT (B). Cells were transiently transfected with plasmids expressing GFP, GFP-E6, GFP-E6*I and GFP-E6MT. Cells were grown and fixed with 4% paraformaldehyde in PBS on the coverslips at 48 h after transfection. The fluorescent images, phase contrast images and merge fluorescent-phase contrast images are shown. V indicates the pEGFP-C1 vector transfected cells. The scale bar represents 20 μm.