Comparison of the RV2 QPCR assay using different rhadinovirus templates diluted in genomic DNA. The PcyRV2 results were obtained using 1 μg of spleen DNA from baboon, Pcy78404, naturally infected with PcyRV2. The other rhadinovirus DNA templates were diluted in uninfected macaque genomic DNA to yield approximately equivalent CT values. The MneRV2 results were obtained using DNA from purified MneRV2 in macaque genomic DNA. The RRV results were obtained using DNA from spleen of MmuA01111, naturally infected with RRV. The MfaRV2 results were obtained using DNA from spleen of Mfa95044, naturally infected with MfaRV2. The released reporter fluorophore is plotted as a function of the amplification cycle number.
Bruce et al. Virology Journal 2005 2:2 doi:10.1186/1743-422X-2-2