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Soilborne wheat mosaic virus (SBWMV) 19K protein belongs to a class of cysteine rich proteins that suppress RNA silencing

Jeannie Te1, Ulrich Melcher2, Amanda Howard1 and Jeanmarie Verchot-Lubicz1*

Author Affiliations

1 Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK 74078, USA

2 Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA

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Virology Journal 2005, 2:18  doi:10.1186/1743-422X-2-18

Published: 1 March 2005

Abstract

Amino acid sequence analyses indicate that the Soilborne wheat mosaic virus (SBWMV) 19K protein is a cysteine-rich protein (CRP) and shares sequence homology with CRPs derived from furo-, hordei-, peclu- and tobraviruses. Since the hordei- and pecluvirus CRPs were shown to be pathogenesis factors and/or suppressors of RNA silencing, experiments were conducted to determine if the SBWMV 19K CRP has similar activities. The SBWMV 19K CRP was introduced into the Potato virus X (PVX) viral vector and inoculated to tobacco plants. The SBWMV 19K CRP aggravated PVX-induced symptoms and restored green fluorescent protein (GFP) expression to GFP silenced tissues. These observations indicate that the SBWMV 19K CRP is a pathogenicity determinant and a suppressor of RNA silencing.