Table 1

Sequences of primers and probes used in real-time qPCR assay and cDNA synthesis
Gene Sequences Amplification product (bp) GeneBank accession number
18S rRNA (F) 5′-CggAggTTCgAAgACgATCA 90 NR_003286
(R) 5′-ggCgggTCATgggAATAAC
(P) 5′(HEX) ATACCgTCgTAgTTCCgACCA (BHQ)
Nucleocapsid (F) 5′-ACCAgAATggAggACgCAATg 85 AY278491
(R) 5′-gCTgTgAACCAAgACgCAgTATTAT
(P) 5′(FAM) ACCCCAAggTTTACCC (NFQ)
ORF1b (F) 5′-CAgAACgCTgTAgCTTCAAAAATCT 68 AY278491
(R) 5′-TCAgAACCCTgTgATgAATCAACAg
(P) 5′(FAM) TCTgCGTAggCAATCC (NFQ)

F: forward primer; R: reverse primer; P: TaqMan probes; 18S rRNA: 18S ribosomal RNA.

Yip et al.

Yip et al. Virology Journal 2014 11:82   doi:10.1186/1743-422X-11-82

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