Nucleocapsid and ORF1b gene expression in SARS-CoV infected human macrophages. Monocyte-derived macrophages from three independent donors were infected with SARS-CoV as described in the legend to Figure 2 in the presence of anti-Spike (black bars) or control serum (white bars). Positive and negative RNA strands were determined for SARS-CoV nucleocapsid (A) and ORF1b (B) at the specified time points (hours) post infection (h.p.i.) using real-time qPCR. Data are shown as the number of viral RNA copies normalized to that of 108 copies of 18S rRNA in the corresponding sample. Overall, RNA levels were higher in infected cells in presence of anti-Spike compared to control serum. Results are shown as means ± SEM of four measurements from two separate runs for each donor and gene.
Yip et al. Virology Journal 2014 11:82 doi:10.1186/1743-422X-11-82