Table 2

Oligonucleotide primers used for RT-LAMP assay
Primer name Sequence (5′–3′) Genome position
FIP = F1c + F2 TTCAGGAATCCCTGAAGGTG-ATCGTCGGMGAGGAVAAGG F1c,203–222, F2,167–185
BIP = B1c + B2 GCGMGAGCTGCCGCCCT-TCTTCVTCAGATCCCCGG B1c,236–252, B2,292–309
F3 AGTTBTGCACGCTCGACAAT 135–154
B3 GTCGACTCTTTGGMGCAATA 320–339
LoopF AGGYGTVCCVTTCGCGC 186–202
LoopB AGGAAGAGCCTGGCTCTC 268–285

Genome position according to the DuCV complete genome sequence (GenBank accession no. DQ100076).

Abbreviations are as follows: M = A or C; V = A or C or G; B = T or G or C.

Xie et al.

Xie et al. Virology Journal 2014 11:76   doi:10.1186/1743-422X-11-76

Open Data