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Open Access Highly Accessed Short report

Plaque reduction neutralization antibody test does not accurately predict protection against dengue infection in Ratchaburi cohort, Thailand

Chukiat Sirivichayakul1*, Arunee Sabchareon1, Kriengsak Limkittikul1 and Sutee Yoksan2

Author Affiliations

1 Department of Tropical Pediatrics, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand

2 Center for Vaccine Development, Mahidol University, Nakhonpathom, Thailand

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Virology Journal 2014, 11:48  doi:10.1186/1743-422X-11-48

Published: 12 March 2014

Abstract

Background

The plaque reduction neutralization test (PRNT) is currently the best and most widely accepted approach to measuring virus-neutralizing and protective antibodies to dengue virus, and in assessing the immunogenicity of a dengue vaccine. However, the correlation between presence of dengue-neutralizing antibody and protection from infection is not absolute.

Findings

In a cohort study in Ratchaburi Province, Thailand, 48 subjects with serologically confirmed symptomatic dengue infection were tested for pre-existing dengue neutralizing antibody using PRNT. Nine subjects had quite high pre-existing PRNT50 titers (titer >90) to subsequent infecting dengue serotypes, but still had symptomatic infections.

Conclusion

This report provides evidence that PRNT may not be a good test for predicting protection against subsequent dengue infection.

Keywords:
Dengue; Plaque reduction neutralization test; Neutralizing antibody