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Genetic characterization of type 2a canine parvoviruses from Taiwan reveals the emergence of an Ile324 mutation in VP2

Chao-Nan Lin12*, Chi-Hsien Chien12, Ming-Tang Chiou1, Ling-Ling Chueh3, Meng-Yu Hung1 and Han-Siang Hsu14

Author Affiliations

1 Department of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung, Taiwan

2 Veterinary Hospital, National Pingtung University of Science and Technology, Pingtung, Taiwan

3 Graduate Institute of Veterinary Medicine, National Taiwan University, Taipei, Taiwan

4 Don-Da Animal Hospital, Taichung, Taiwan

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Virology Journal 2014, 11:39  doi:10.1186/1743-422X-11-39

Published: 25 February 2014



Canine parvovirus 2 (CPV 2) is a major infectious cause of mortality in puppies. The characteristic symptom of CPV 2 disease is intestinal hemorrhage with severe bloody diarrhea. Soon after CPV was first recognized in the late 1970s, the original virus, CPV 2, was replaced in the canine population by strains carrying minor antigenic variants (termed 2a, 2b, and 2c) of the VP2 gene that could be distinguished using monoclonal antibodies and molecular analyses. Here, we provide an updated molecular characterization of the CPV 2 circulating in Taiwan.


In this study, 28 isolates of CPV 2 from 144 dogs with suspected CPV infection were obtained from northern, central, and southern Taiwan from 2008 to 2012 and screened by PCR. The 28 isolates were sequenced, and a phylogenetic analysis of the VP2 gene was performed.


Of the 28 Taiwanese CPV 2 isolates, 15 were identified as new CPV 2a, and 13 were identified as new CPV 2b. Compared to the reference CPV 2a, all 15 of the CPV 2a sequences collected in this study contain an Ile324 mutation caused by a TAT to ATT mutation at nucleotides 970–972 of the VP2 gene.


Our VP2 sequence data revealed that both types are currently prevalent CPV 2 field strains circulating in Taiwan, and a unique Ile324 VP2 mutation was found in our Taiwanese CPV 2a isolates and recent Asian isolates. CPV 2c was not observed in this study.

Canine parvovirus; Genotype; VP2; Sequence analysis