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Evaluation of respiratory syncytial virus group A and B genotypes among nosocomial and community-acquired pediatric infections in southern Brazil

Fernanda de-Paris13*, Caroline Beck2, Luciana de Souza Nunes1, Alice Beatriz Mombach Pinheiro Machado3, Rodrigo Minuto Paiva3, Denise da Silva Menezes3, Márcia Rosane Pires4, Rodrigo Pires dos Santos4, Ricardo de Souza Kuchenbecker2 and Afonso Luis Barth13

Author Affiliations

1 Universidade Federal do Rio Grande do Sul (UFRGS), Faculdade de Medicina, Programa de Pós-graduação em Ciências Médicas, Rua Ramiro Barcelos 2400, Porto Alegre, RS, Brazil

2 Instituto de Avaliação de Tecnologia em Saúde/IATS/CNPq, Universidade Federal do Rio Grande do Sul (UFRGS), Faculdade de Medicina, Programa de Pós-graduação em Epidemiologia, Rua Ramiro Barcelos 2400, Porto Alegre, RS, Brazil

3 Hospital de Clínicas de Porto Alegre (HCPA), Serviço de Patologia Clínica, Unidade de Pesquisa Biomédica–Laboratório de Biologia Molecular, Unidade de Microbiologia e Biologia Molecular, Rua Ramiro Barcelos 2350, Porto Alegre, RS, Brazil

4 Hospital de Clinicas de Porto Alegre (HCPA), Comissão de Controle de Infecção Hospitalar, Rua Ramiro Barcelos 2350, Porto Alegre, RS, Brazil

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Virology Journal 2014, 11:36  doi:10.1186/1743-422X-11-36

Published: 24 February 2014

Abstract

Background

Respiratory syncytial virus (RSV) is the main cause of lower respiratory tract illness in children worldwide. Molecular analyses show two distinct RSV groups (A and B) that comprise different genotypes. This variability contributes to the capacity of RSV to cause yearly outbreaks. These RSV genotypes circulate within the community and within hospital wards. RSV is currently the leading cause of nosocomial respiratory tract infections in pediatric populations. The aim of this study was to evaluate the G protein gene diversity of RSV amplicons.

Methods

Nasopharyngeal aspirate samples were collected from children with nosocomial or community-acquired infections. Sixty-three RSV samples (21 nosocomial and 42 community-acquired) were evaluated and classified as RSV-A or RSV-B by real-time PCR. Sequencing of the second variable region of the G protein gene was performed to establish RSV phylogenetics.

Results

We observed co-circulation of RSV-A and RSV-B, with RSV-A as the predominant group. All nosocomial and community-acquired RSV-A samples were from the same phylogenetic group, comprising the NA1 genotype, and all RSV-B samples (nosocomial and community-acquired) were of the BA4 genotype. Therefore, in both RSV groups (nosocomial and community-acquired), the isolates belonged to only one genotype in circulation.

Conclusions

This is the first study to describe circulation of the NA1 RSV genotype in Brazil. Furthermore, this study showed that the BA4 genotype remains in circulation. Deciphering worldwide RSV genetic variability will aid vaccine design and development.

Keywords:
Respiratory syncytial virus; Nosocomial infection; G-protein; Genetic variability; Molecular epidemiology