Phylogenetic tree based on the proteomes of 24 N4-like phages. The tree was inferred from pairwise distances, assessed via the GBDP approach [57-59], and using the balanced minimum evolution principle . The tree was rooted via midpoint rooting . GBDP used BLAST +  as a local alignment programme (i.e., blastp using an initial word length of 3) and applied the following settings for the pairwise distance calculation: trimming algorithm, formula d5 and no e-value filter.
Wittmann et al. Virology Journal 2014 11:14 doi:10.1186/1743-422X-11-14