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Open Access Research

Chemiluminescent microparticle immunoassay based detection and prevalence of HCV infection in district Peshawar Pakistan

Muhammad Ilyas1 and Irshad Ahmad2*

Author Affiliations

1 Department of Microbiology and Biotechnology, Sarhad University of Science and Information Technology (SUIT), 25120 Peshawar, Pakistan

2 Biology Department, King Fahd University of Petroleum and Minerals (KFUPM), 34464 Dhahran, Saudi Arabia

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Virology Journal 2014, 11:127  doi:10.1186/1743-422X-11-127

Published: 12 July 2014

Abstract

Background

Due to the high rate of asymptomatic infections an advanced screening assay is of prompt importance to be used for the clinical diagnosis of HCV. Early detection of anti HCV is the first step in the management of chronic hepatitis and in the selection of patients needing treatments. In the current study we have first time used the advanced serological diagnostic technique i.e. Chemiluminescent Microparticle Immuno Assay (CMIA) for the detection of HCV infection in Peshawar Pakistan.

Methods

A total number of 982 samples were collected among the general public belongs to the different areas of district Peshawar. The samples were centrifuged at high speed to obtain a clear supernatant serum. All the samples were run on Architect system a fully automated immuno analyzer CMIA base technology.

Results

Out of 982 blood samples analyzed in this study, 160 (15.9%) were confirmed to be positive for active HCV infection. The overall prevalence was found to be 13.4%. Gender wise prevalence was recorded to be higher in male (19.1%) than female (12.7%). The age group 21-30 years was identified as the highest risk group among the studied population.

Conclusion

Among the tested samples, overall prevalence of active HCV infection was found to be 13.4% in the general population of Peshawar Pakistan. The young middle aged population of this region was at higher risk of HCV ailments compared to the other age groups.

Keywords:
HCV; CMIA; Prevalence