Additional file 5: Figure S5.

Phosphorylation of H2AX by Vpr. HeLa cells (1 x 105) were transfected with 0.1 μg of HA-Vpr or SIVagm HA-Vpr expression vector. Twenty-four hours after transfection, the cells were lysed in sample buffer. Vpr and γH2AX in the cell lysate were detected by immunoblot analysis. The βtubulin was a loading control.

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Hakata et al. Virology Journal 2014 11:108   doi:10.1186/1743-422X-11-108