Additional file 4: Figure S4.

SIVagm HA-Vpr shows a defect in UNG2 degradation. 293T cells were transfected with increasing amounts (0.05 μg, 0.1 μg, and 0.2 μg) of HA-Vpr or SIVagm HA-Vpr expression vector together with a constant amount of HA-UNG2 and Flag-DCAF1 expression vectors. The cells were lysed, and then Vpr and UNG2 were detected by immunoblot analysis with anti-HA antibody. The βtubulin was a loading control.

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Hakata et al. Virology Journal 2014 11:108   doi:10.1186/1743-422X-11-108