Additional file 3: Figure S3.

Colocalization of VprR90K with DDB1. HeLa cells were transfected with HA-Vpr or HA-VprR90K and Flag-DCAF1 expression vectors. The transfected cells were permeabilized, fixed, and then incubated with anti-DDB1 and anti-HA antibodies followed by Alexa Fluor 594-anti-rabbit IgG and Alexa Fluor 488-anti-rat IgG. The percentage of Vpr foci colocalized with DDB1 among total Vpr foci was calculated. More than 380 Vpr foci were evaluated for each sample and three independent experiments were done. The data are the mean values with standard deviations. P values were calculated by the Student’s t-test with P < 0.05 considered significant.

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Hakata et al. Virology Journal 2014 11:108   doi:10.1186/1743-422X-11-108