Additional file 2: Figure S2.

Cell cycle analysis of VprR90K and VprR90D mutants. (A) 293T cells were transfected with HIV-1 Vpr or Vpr mutant, Flag-DCAF1, and EGFP expression vectors. After staining with propidium iodide, the cells were analyzed by flow cytometry. The G2:G1 ratio was calculated after gating for the GFP+ cells. The results are representative of three independent experiments. (B) A portion of the cells used in (A) was subjected to an immunoblot analysis to confirm Vpr and Vpr mutants expression. The ╬▓tubulin was a loading control.

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Hakata et al. Virology Journal 2014 11:108   doi:10.1186/1743-422X-11-108