Additional file 1: Figure S1.

Association of VprQ65R mutant with DCAF1 and DDB1. 293T cells were cotransfected with HA-Vpr or HA-VpQ65R and Flag-DCAF1 expression vectors. Vpr was immunoprecipitated with anti-HA antibody and the immunoprecipitates were subjected to immunoblot analysis with anti-Flag MAb, anti-DDB1 antibody, and anti-HA antibody.

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Hakata et al. Virology Journal 2014 11:108   doi:10.1186/1743-422X-11-108