Table 3

Real-time quantitative RT-PCR analysis of the inhibition of FMDV replication in pEGFP-miR242 + 276-transformed BHK-21 cells, compared with normal BHK-21 cells
FMDV Ct values (mean)
12 h.p.i. 24 h.p.i. 36 h.p.i. 48 h.p.i. 60 h.p.i. 72 h.p.i.
O/Tibet/China/1/99 34.61 ± 0.32/37.73 ± 0.07 36.20 ± 0.09/38.76 ± 0.25 35.98 ± 0.83/36.09 ± 0.12 37.55 ± 0.22/36.75 ± 0.44 37.62 ± 0.51/26.92 ± 0.21 36.08 ± 0.08/28.49 ± 0.39
O/HN/CHA/93 36.45 ± 0.05/35.65 ± 0.66 36.85 ± 0.57/31.32 ± 0.10 33.41 ± 0.15/28.62 ± 0.40 28.54 ± 0.10/28.82 ± 0.49 26.84 ± 0.16/26.34 ± 0.11 26.34 ± 0.30/26.54 ± 0.22
O/CHN/Mya98/33-P 16.70 ± 0.10/16.27 ± 0.23 15.17 ± 0.02/14.57 ± 0.04 11.81 ± 0.30/12.44 ± 0.14 8.43 ± 0.59/14.16 ± 0.41 7.49 ± 0.31/9.16 ± 0.04 13.68 ± 0.10/15.02 ± 0.59
AF72 30.97 ± 0.08/25.23 ± 0.17 36.23 ± 0.78/30.44 ± 0.41 36.36 ± 0.18/35.02 ± 0.79 38.45 ± 1.07/25.21 ± 0.92 36.32 ± 0.94/10.32 ± 0.71 37.13 ± 0.15/11.16 ± 0.31
Asia1/Jiangsu/China/2005 36.73 ± 0.18/34.16 ± 0.68 37.12 ± 0.11/35.65 ± 0.19 37.28 ± 0.39/35.46 ± 0.04 35.42 ± 0.09/27.39 ± 0.20 36.62 ± 0.57/17.84 ± 0.63 38.59 ± 0.77/16.68 ± 0.44

Viral supernatants were collected at 12, 24, 36, 48, 60, and 72 h.p.i., and the Ct values were derived from two parallel experiments (treatment/control).

Chang et al.

Chang et al. Virology Journal 2014 11:1   doi:10.1186/1743-422X-11-1

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