|Efficiencies of miRNAs targeting the FMDV IRES in inhibiting EGFP expression in BHK-21 cells as assayed by flow cytometry|
|Reporter plasmid||Inhibition efficiency of each miRNA (%)|
Bi-miRNA, a mixture of pmiR242 and pmiR276 plasmids; Dual-miRNA, a co-cistronic expression plasmid (pmiR242 + 276) containing two pre-miRNA (pmiR242 and pmiR276) hairpin structures. Percentage inhibition in each co-transfected (vector-delivered miRNAs and the reporter plasmids) cell population was calculated by comparison with the control cells transfected only with the same reporter plasmid at 48 h post-transfection.
Chang et al.
Chang et al. Virology Journal 2014 11:1 doi:10.1186/1743-422X-11-1