Schematic representations of (A) FMDV-specific miRNAs and (B) IRES-EGFP expression plasmids. 5′ and 3′ miR flanking regions are represented as grey. Procedures for the construction of single miRNA (pmiR153, pmiR220, pmiR242, and pmiR276), Dual-miRNAs (pmiR242 + 276 and pEGFP-miR242 + 276) and the reporter plasmids (pHN/IRES-EGFP, pFC/IRES-EGFP, and pJS/IRES-EGFP) are described in Methods.
Chang et al. Virology Journal 2014 11:1 doi:10.1186/1743-422X-11-1