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Open Access Highly Accessed Research

The host-range, genomics and proteomics of Escherichia coli O157:H7 bacteriophage rV5

Andrew M Kropinski12, Tom Waddell3, Juncai Meng4, Kristyn Franklin1, Hans-Wolfgang Ackermann5, Rafiq Ahmed6, Amanda Mazzocco1, John Yates7, Erika J Lingohr1 and Roger P Johnson1*

Author Affiliations

1 Public Health Agency of Canada, Laboratory for Foodborne Diseases, 110 Stone Road West, Guelph, ON N1G 3W4, Canada

2 Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, N1G 2W1, Canada

3 Abbott Point of Care, 185 Corkstown Road, Ottawa, ON, K2H 8V4, Canada

4 Merck Research Laboratories, 126E Lincoln Avenue, Rahway, NJ, 07065, USA

5 Département de Microbiologie-infectiologie et immunologie, Faculté de médecine, Université Laval, Québec, QC, G1K 7P4, Canada

6 Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB, R3E 3R2, Canada

7 The Scripps Research Institute, Department of Cell Biology, Proteomic Mass Spectrometry Laboratory, 10550 North Torrey Pines Road, La Jolla, CA, 92037, USA

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Virology Journal 2013, 10:76  doi:10.1186/1743-422X-10-76

Published: 6 March 2013

Abstract

Background

Bacteriophages (phages) have been used extensively as analytical tools to type bacterial cultures and recently for control of zoonotic foodborne pathogens in foods and in animal reservoirs.

Methods

We examined the host range, morphology, genome and proteome of the lytic E. coli O157 phage rV5, derived from phage V5, which is a member of an Escherichia coli O157:H7 phage typing set.

Results

Phage rV5 is a member of the Myoviridae family possessing an icosahedral head of 91 nm between opposite apices. The extended tail measures 121 x 17 nm and has a sheath of 44 x 20 nm and a 7 nm-wide core in the contracted state. It possesses a 137,947 bp genome (43.6 mol%GC) which encodes 233 ORFs and six tRNAs. Until recently this virus appeared to be phylogenetically isolated with almost 70% of its gene products ORFans. rV5 is closely related to coliphages Delta and vB-EcoM-FY3, and more distantly related to Salmonella phages PVP-SE1 and SSE-121, Cronobacter sakazakii phage vB_CsaM_GAP31, and coliphages phAPEC8 and phi92. A complete shotgun proteomic analysis was carried out on rV5, extending what had been gleaned from the genomic analyses. Host range studies revealed that rV5 is active against several other E. coli.

Keywords:
E. coli O157:H7; Phage evolution; Phage ecology; Myoviridae; Genomics; Proteomics; Bioinformatics; Pyrosequencing; Tail spike