Sequestration of virus proteins in Arf6/Q67L induced PI(4,5)P2-enriched vesicles. Immunofluorescence microscopy of HeLa cells expressing HA-tagged Arf6/Q67L and PH-GFP followed by BTV-1 infection. The infected cells were analyzed 12hrs post-infection for localization of NS3 (B) and VP5 (C). Control consists of cells that have been co-transfected with PH-GFP and HA-tagged Arf6/Q67L, but not infected (A). While PH-GFP localization is shown in green, HA-tagged Arf6/Q67L was detected with Alexa Fluor (blue) and all the viral proteins with TRITC (red). White circles point to co-localized patches. The scale has been included in the merged panel of each row.
Bhattacharya and Roy Virology Journal 2013 10:73 doi:10.1186/1743-422X-10-73