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Comparison of host cell gene expression in cowpox, monkeypox or vaccinia virus-infected cells reveals virus-specific regulation of immune response genes

Daniel Bourquain1, Piotr Wojtek Dabrowski12 and Andreas Nitsche1*

  • * Corresponding author: Andreas Nitsche nitschea@rki.de

  • † Equal contributors

Author Affiliations

1 Centre for Biological Threats and Special Pathogens 1, Robert Koch Institute, Nordufer 20, Berlin 13353, Germany

2 Central Administration 4 (IT), Robert Koch Institute, Nordufer 20, Berlin 13353, Germany

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Virology Journal 2013, 10:61  doi:10.1186/1743-422X-10-61

Published: 20 February 2013

Additional files

Additional file 1:

Overview of changes in transcript expression following infection. Scatter plot of signal intensities of all spots. Data was obtained by merging the data sets of two replicates. The plots illustrate a comparison of signal intensities from non-infected cells versus CPXV- (A), MPXV- (B) or VACV- (C) infected cells. The signal intensities of each feature represented by a dot are shown in double logarithmic scale. X-axis: mock signal intensity; y-axis: infected sample signal intensity. Red diagonal lines define areas of 2-fold differential signal intensities. Blue spots define unchanged expression; red dots: transcripts significantly upregulated and green dots: transcripts significantly down-regulated in the infected samples (p-value ≤0.01). The grey cross in the legend marks the number of significantly up- and downregulated genes.

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Additional file 2:

genes regulated by CPXV, MPXV and VACV. Shown is the relative expression of genes which were regulated by infection with all OPV tested to a similar extent. Included are genes that exhibited more than 2-fold up- or downregulation following infection. Red and green colour displays up- or downregulation in the infected cells compared to non-infected cells (shown are 2 arrays per virus). Numbers indicate the log10 of the change fold values.

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Additional file 3:

Distribution of up- or downregulated genes. The figure shows the distribution of up- or downregulated transcripts following infection with CPXV (A), MPXV (B) or VACV (C) in comparison to non-infected cells. The degree of regulation compared to the non-infected control is displayed on the x-axis. A global minor downregulation below the set cut-off value of ≥2 fold change occurred following infection with MPXV and VACV.

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Open Data