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Detection of DENV-4 genotype I from mosquitoes collected in the city of Manaus, Brazil

Mario Luis Garcia de Figueiredo1*, Helda L Alfonso2, Alberto Anastacio Amarilla2, Luiz Tadeu Moraes Figueiredo3, Victor Hugo Aquino2, Cristóvão Alves da Costa4 and Sergio Luiz Bessa Luz4

Author Affiliations

1 Fundação Osvaldo Cruz – FIOCRUZ, Av. Teresina, 476 - Adrianópolis, CEP:(69.057-070, Manaus, AM, Brazil

2 Department of Clinical, Toxicological and Bromatological Analysis, FCFRP/USP, Ribeirão Preto, SP, Brazil

3 Virology Research Center, FMRP/USP, Ribeirão Preto, SP, Brazil

4 National Institute for Research in the Amazon Region Manaus, Amazonas, Brazil

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Virology Journal 2013, 10:60  doi:10.1186/1743-422X-10-60

Published: 19 February 2013



Dengue epidemics have been reported in Brazil since 1981. In Manaus, a large city in the Amazon region, dengue is endemic with all four-virus serotypes (DENV-1, -2, -3, and -4) simultaneously causing human disease. In 2008, during a surveillance of dengue virus in mosquitoes in the district of Tancredo Neves in Manaus, 260 mosquitoes of Aedes genus were captured, identified and grouped into pools of 10 mosquitoes.


RNA extracts of mosquito pools were tested by a RT-Hemi-Nested-PCR for detection of flaviviruses. One amplicon of 222 bp, compatible with dengue virus serotype 4, was obtained from a pool of Aedes aegypti. The nucleotide sequence of the amplicon indicated that the mosquitoes were infected with DENV-4 of genotype I. This virus of Asian origin has been described in Manaus in 2008 infecting acute febrile illness patients.


This is the first report of dengue virus serotype 4 genotype I infecting Aedes aegypti in the Americas.

Emerging flavivirus; Dengue serotype 4 in Brazil; RT-Hemi-Nested-PCR for Flavivirus