Detection of DENV-4 genotype I from mosquitoes collected in the city of Manaus, Brazil
1 Fundação Osvaldo Cruz – FIOCRUZ, Av. Teresina, 476 - Adrianópolis, CEP:(69.057-070, Manaus, AM, Brazil
2 Department of Clinical, Toxicological and Bromatological Analysis, FCFRP/USP, Ribeirão Preto, SP, Brazil
3 Virology Research Center, FMRP/USP, Ribeirão Preto, SP, Brazil
4 National Institute for Research in the Amazon Region Manaus, Amazonas, Brazil
Virology Journal 2013, 10:60 doi:10.1186/1743-422X-10-60Published: 19 February 2013
Dengue epidemics have been reported in Brazil since 1981. In Manaus, a large city in the Amazon region, dengue is endemic with all four-virus serotypes (DENV-1, -2, -3, and -4) simultaneously causing human disease. In 2008, during a surveillance of dengue virus in mosquitoes in the district of Tancredo Neves in Manaus, 260 mosquitoes of Aedes genus were captured, identified and grouped into pools of 10 mosquitoes.
RNA extracts of mosquito pools were tested by a RT-Hemi-Nested-PCR for detection of flaviviruses. One amplicon of 222 bp, compatible with dengue virus serotype 4, was obtained from a pool of Aedes aegypti. The nucleotide sequence of the amplicon indicated that the mosquitoes were infected with DENV-4 of genotype I. This virus of Asian origin has been described in Manaus in 2008 infecting acute febrile illness patients.
This is the first report of dengue virus serotype 4 genotype I infecting Aedes aegypti in the Americas.