A) Inverse PCR was performed to obtain the NTR sequence between the VP termination and the NS initiation codon of episomal forms of CnBoV3. Primers (P1 and P2) were situated at VP and NS region respectively. Secondary structures (symbolic in the box) may have impeded the PCR resulting in several products of different length. B) Highly identical ‘rabbit ear’ structure of CnBoV3 and MVC. C) Alignments of NTR sequences obtained by sequencing different inverse PCR products directly (PCR1&2) and subcloning products (colony 1–4). Sequences outside the two arrows were identical. Underlined sequence shows location of “rabbit ear” sequence.
Li et al. Virology Journal 2013 10:54 doi:10.1186/1743-422X-10-54