Short report
A novel bocavirus in canine liver
1 Blood Systems Research Institute, San Francisco, CA, USA
2 Department of Laboratory Medicine, University of California, San Francisco, CA, USA
3 Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA, USA
4 IDEXX Reference Laboratories, CA, USA
5 UCSF-Abbott Viral Diagnostics and Discovery Center, CA, USA
6 Department of Microbiology, University of Kansas, KS, USA
7 Stanford Genome Technology Center, Stanford, CA, USA
Virology Journal 2013, 10:54 doi:10.1186/1743-422X-10-54
Published: 13 February 2013Abstract
Background
Bocaviruses are classified as a genus within the Parvoviridae family of single-stranded DNA viruses and are pathogenic in some mammalian species. Two species have been previously reported in dogs, minute virus of canines (MVC), associated with neonatal diseases and fertility disorders; and Canine bocavirus (CBoV), associated with respiratory disease.
Findings
In this study using deep sequencing of enriched viral particles from the liver of a dog with severe hemorrhagic gastroenteritis, necrotizing vasculitis, granulomatous lymphadenitis and anuric renal failure, we identified and characterized a novel bocavirus we named Canine bocavirus 3 (CnBoV3). The three major ORFs of CnBoV3 (NS1, NP1 and VP1) shared less than 60% aa identity with those of other bocaviruses qualifying it as a novel species based on ICTV criteria. Inverse PCR showed the presence of concatemerized or circular forms of the genome in liver.
Conclusions
We genetically characterized a bocavirus in a dog liver that is highly distinct from prior canine bocaviruses found in respiratory and fecal samples. Its role in this animal’s complex disease remains to be determined.
