Schematic rVSV NiVB vaccine genomes and groups. (A) Group 1: rVSV-ΔG-GFP expressing no glycoprotein; produces ‘bald’ virions, Group 2 GInd*rVSV-NiVB /F-GFP expressing the NiVB F protein (blue); produces virions with F on the surface, Group 3 GInd*rVSV-NiVB /G-GFP expressing the NiVB G protein (yellow); produces virions with G on the surface, Group 4 made from co-infection in Vero cells resulting in a combination of rVSV-NiVB /F-GFP and rVSV-NiVB /G-GFP virions expressing the NiVB F and G proteins in the same cells; produces single-cycle infectious virions with NiVB F and G proteins on the cell surface (green). (B) Phase and fluorescence microscopy of Vero cells infected with virions from Group 4 displaying multinucleated syncytia cells * and cells positive for GFP expression.
Mire et al. Virology Journal 2013 10:353 doi:10.1186/1743-422X-10-353