Flow chart showing the organization of HPV and EBV detection and expression in squamous cell carcinoma (SCC) cases by PCR using MY consensus primers and by Digene Hybrid Capture II (HC II). The Epstein-Barr virus (EBV) was detected by PCR using primers that amplify the entire BALF-1 gene. EBV expression was investigated by LMP-1 Reverse Transcription-PCR, BARF-1 Immunoblotting and LMP-1 and EBNA-1 Immunohistochemistry.
Khenchouche et al. Virology Journal 2013 10:340 doi:10.1186/1743-422X-10-340